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Every 24 h, medium change was performed by centrifugation of cells at 900 r.p.m

doi: 10.1093/ilar.53.3-4.289 49 ZolotaVTzelepiVPiperigkouZKoureaHPapakonstantinouEArgentouM-Iet al

After 18 h incubation at 27C, excess media was removed from the wells and replaced with 100 L of solution C containing either 9 10 3 (1 MOI) or 2.7 10 4 (3 MOI) zoospores per well for 1 hr, after which the inoculation solution as removed and replaced with 100 L solution A (25, 28)

PLoS ONE 4 , e7754 (2009)

In addition to the observed anti-inflammatory effects of the BCAAs in vitro and in mouse models, this group of AAs also has demonstrated anti-inflammatory effects in immune compromised humans

But if you are going to make a case for selling salt water as a rejuvenation therapy, you have to come up with something a bit more impressive than advancing life. So what claim did ASEA come up with